Synthesis and functional characterization of a recombinant HIV-1 reverse transcriptase variant carrying twelve drug-resistance mutations as a platform for antiretroviral screening

Key Points

• Novel HIV-1 reverse transcriptase variant exhibits twelve drug-resistance mutations, impacting antiretroviral efficacy.
• Functional characterization demonstrates altered enzyme activity, revealing potential challenges in treatment protocols.
• Synthesis of the recombinant variant provides a useful platform for further antiretroviral drug screening and evaluation.
• Recognizing drug resistance is crucial for optimizing treatment strategies; ongoing research may enhance patient outcomes.