Pseudomonas aeruginosa is a highly adaptable opportunistic pathogen with a repertoire of mechanisms for surviving in diverse and often challenging environments, yet it is most studied at 37°C as the optimum temperature for growth. To better understand how this bacterium survives in the environment versus the human body, we performed transcriptomics on P. aeruginosa grown at 25°C and 37°C. At each temperature, we examined both exponential and stationary phases and determined the LasRI quorum sensing regulon at 37°C compared to 25°C using a ΔlasR mutant, which uncovered a suite of previously unrecognized LasR-regulated genes. Our work provides a comprehensive transcriptomic resource for the thermoregulation of P. aeruginosa at two growth phases, as well as growth phase and LasR regulation at two temperatures.
Robinson et al. (Wed,) studied this question.