To investigate how ISGylation of GAPDH influences glycolytic control and energy metabolism.
Used proteomics techniques to analyze metabolic pathways
Assessed the role of ISG15 in glycolytic regulation
Investigated the post-translational modification mechanisms of GAPDH
Identified GAPDH as a key metabolic checkpoint
Established a link between ISG15 and glycolytic control
Revealed post-translational mechanisms regulating energy metabolism
Abstract
These findings identify GAPDH as a central metabolic checkpoint regulated by ISGylation and uncover a direct post-translational mechanism by which ISG15 controls energy metabolism.