What is the clinical evidence from this study?

Study design: Other. Population: Peripheral Artery Disease (n=154). Intervention: FBXW7, MASP1, and SERP1 mRNA Expression vs. Healthy individuals. Primary outcome: mRNA expression levels of FBXW7, MASP1, and SERP1 (p=>0.05).

What does this research mean for the field?

FBXW7, MASP1, and SERP1 mRNA expression levels do not differ significantly between patients with Peripheral Artery Disease and healthy individuals. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

Evaluate the roles of FBXW7, MASP1, and SERP1 as potential biomarkers in patients with Peripheral Artery Disease.

March 16, 2026Open Access

Investigation of FBXW7, MASP1 and SERP1 mRNA Expression Levels in Patients with Peripheral Artery Disease

Key Result

FBXW7, MASP1, and SERP1 mRNA expression levels did not differ significantly between patients with peripheral artery disease and healthy controls (p > 0.05).

Key Points

Evaluate the roles of FBXW7, MASP1, and SERP1 as potential biomarkers in patients with Peripheral Artery Disease.
Analyzed mRNA expression of FBXW7, MASP1, and SERP1 in blood samples.
Included 78 patients with Peripheral Artery Disease and 76 healthy individuals.
Utilized real-time polymerase chain reaction (rt-PCR) for gene expression measurement.
No significant differences in gene expression levels were found between PAD patients and healthy individuals (p > 0.05).
FBXW7, MASP1, and SERP1 do not seem to directly contribute to the development of PAD.

Structured PICO

Are mRNA expression levels of FBXW7, MASP1, and SERP1 altered in patients with peripheral artery disease compared to healthy controls?

Population

154 adults aged 18-80 years, including 78 patients with symptomatic Peripheral Artery Disease (intermittent claudication, Rutherford Category 2-4) and 76 age- and sex-matched healthy volunteers with no clinical history of vascular disease.

Comparator

Healthy individuals with no clinical history of vascular disease and normal vascular flow confirmed by Doppler USG.

Outcome

mRNA expression levels of FBXW7, MASP1, and SERP1 genes in blood samples, determined using real-time polymerase chain reaction (rt-PCR).surrogate

FBXW7, MASP1, and SERP1 mRNA expression levels do not appear to be significantly altered in peripheral artery disease, suggesting they may not serve as primary diagnostic biomarkers for this condition.

Main Result

p-value: p=>0.05

Limitations

Lack of longitudinal data to track expression changes over time.
Results should be interpreted as non-significant trends.

Abstract

Objective: Peripheral Artery Disease (PAD) is the third leading cause of cardiovascular morbidity worldwide. Given their established roles in lipid metabolism, the lectin-complement pathway, and endoplasmic reticulum stress, we aimed to investigate the potential involvement of the FBXW7, MASP1, and SERP1 genes in PAD. This study sought to evaluate whether these genes could serve as non-invasive biomarkers for the diagnosis of PAD, as they have not been previously explored in this patient population. Materials and Methods: A total of 78 individuals with peripheral arterial disease and 76 healthy individuals were included in the study. Total RNA was extracted from blood samples, and the expressions of FBXW7, MASP1, and SERP1 mRNAs were determined using the real-time polymerase chain reaction (rt-PCR). Results: No statistically significant differences were observed in the expression levels of FBXW7, MASP1, and SERP1 genes between PAD patients and healthy subjects (p 0.05). Conclusion: Although FBXW7, MASP1, and SERP1 proteins are associated with arterial function, they do not appear to play a direct role in the development of peripheral artery disease.

Bookmark

View Full Paper