We report optimisation of isolation and purification of exosomes derived from bone marrow mesenchymal stem cells, characterising their composition and functional properties using advanced techniques like ultracentrifugation for exosome isolation, flow cytometry, Fourier transform infrared spectroscopy (FTIR), zeta potential, miR-122-5p expression, and protein quantification via Bradford assay. Exosomes ranged between 154 and 250 nm in size. Flow cytometry confirmed presence of characteristic surface markers, including CD73, CD90, and CD105. Additionally, FTIR analysis provided detailed molecular insights, confirming the presence of miR-122-5p within exosomal cargo. This study underscores the need for standardised methodologies in exosome QC given their promise in therapeutic applications.
Kazmi et al. (Wed,) studied this question.