Mechanism study of italicTaohong Siwu Decoction/italic italicJiawei /italic（桃红四物汤加味） in regulating Th17/Treg balance and ovarian estradiol secretion for psoriasis treatment

Key Points

• The study aims to explore the effects of Taohong Siwu Decoction on Th17/Treg balance and estradiol secretion in psoriasis.
• Utilized 72 SPF female BALB/c mice divided into different treatment groups.
• Induced psoriasis-like skin lesions using 5% imiquimod cream.
• Applied Taohong Siwu Decoction at low, medium, and high doses during intervention.
• Employed techniques like HE staining, TUNEL, IHC, ELISA, flow cytometry, Western blot, and real-time PCR.
• PASI scores, Baker scores, and apoptosis rates of ovarian granulosa cells were significantly decreased in treated groups.
• IL-17 levels were reduced, while IL-2, IL-10, and estradiol levels increased.
• Increased expression levels of P450scc, 3β-HSD, and 17β-HSD mRNA were observed.
• Th17+ cell positivity and RORγt protein expression decreased, while Treg+ cell positivity and Foxp3, ERα, ERβ expression increased.
• The Th17/Treg cell ratio was significantly reduced.