What question did this study set out to answer?

The research aimed to examine how fatty acids, particularly eicosadienoic acid, affect antibody production in CHO cells.

April 8, 2026Open Access

Eicosadienoic acid enhances antibody production in CHO cells by prolonging culture longevity and attenuating apoptosis

Key Points

The research aimed to examine how fatty acids, particularly eicosadienoic acid, affect antibody production in CHO cells.
Investigated the effect of various fatty acids on antibody expression in CHO cells.
Used batch and fed-batch culture systems for testing eicosadienoic acid supplementation.
Measured antibody yield and assessed apoptosis and culture duration changes.
Eicosadienoic acid increased antibody yield by 48% in batch culture and 59% in fed-batch culture.
Culture longevity was significantly extended with EDA addition.
Apoptosis rates in the cultures were reduced.
EDA also enhanced yields of other antibodies like Rituximab and Herceptin.

Abstract

Fatty acids are key nutrients exerting multiple functions in cells. This study aimed to investigate the effect of fatty acids on antibody expression in Chinese hamster ovary (CHO) cells. Among different fatty acids, cis, cis − 11, 14-eicosadienoic acid (EDA, C20: 2, 11, 14-cis) was screened to exert the strongest effect to boost antibody yield in CHO-IgG cells tested. In batch and fed-batch culture processes, the antibody yield was promoted by 48% and 59% with supplementation of 10 µM of EDA respectively. Meanwhile, the culture duration was significantly prolonged while the apoptosis ratios were suppressed by EDA. The addition of EDA also promoted the yield of other antibodies transiently expressed in CHO-K1 cells, including Rituximab, Herceptin, and one anti-uPAR antibody. These findings revealed the potential of EDA in promoting antibody expression in CHO cells, shedding light on the application of functional fatty acids in therapeutic antibody manufacturing.

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Cite This Study

Pei et al. (Sun,) studied this question.

synapsesocial.com/papers/69d5efd374eaea4b11a79670 https://doi.org/https://doi.org/10.1186/s13568-026-02050-4

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