Key points are not available for this paper at this time.
This protocol offers an Image J macro/plugin that enable easy quantification of bands on western blots, dot blots, and fluorescent gels etc., by simply selecting bands with rectangle or oval tools as region of interest (ROI), and running this macro. This macro automatically estimates background level based on mean or median intensity around ROI boundary, and should help you speed up your routine quantification tasks. Note that the quantification algorithm is similar to that used in Image Studio Lite software (freely available from LI-COR Biosciences), which is widely used for quantification purpose in many publications.
Ohgane et al. (Tue,) studied this question.