This study employed lipidomics technology based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) to systematically analyze the effects of flaxseed-supplemented feed on the phospholipid composition of egg yolk in laying hens. The results indicate that flaxseed intervention significantly increases the content of neuro-related glycosphingolipids in egg yolk, with galactosylceramide increasing by approximately 1.67-fold, glucosylceramide by 2.00-fold, and ganglioside GM3 by 3.00-fold, while also promoting the specific enrichment of ω-3 polyunsaturated fatty acids at the sn-1 position of phospholipids. Additionally, the levels of plasmalogens, which possess antioxidant functions, are notably elevated. These changes collectively optimize the nutritional structure of egg yolk phospholipids, demonstrating that flaxseed-supplemented feed can transform egg yolk into a composite lipid system with potential neuroprotective functions, thereby providing a new scientific basis for the development of functional egg products. • Glycosphingolipids were detected and enriched by flaxseed feeding. • ω-3 PUFAs were esterified at the sn-1 position of phosphatidylcholine. • Flaxseed feeding enriched plasmalogens in egg yolk. • Flaxseed feeding enriched glycosphingolipids and phospholipids in egg yolk.
Wang et al. (Wed,) studied this question.