An acne treatment patch was developed by incorporating Rhodomyrtus tomentosa (RT) leaf extract into hyaluronic acid serum, followed by integration with high‐quality block natural rubber using a two‐roll milling process. RT hyaluronic acid serum exhibited strong antibacterial activity against pathogens associated with acne vulgaris. The minimum inhibitory concentrations and minimum bactericidal concentrations ranged from 0.39% to 12.5% and 0.39% to 25%, respectively. Acne patches containing RT hyaluronic acid serum (RT patch) were evaluated for their structural, mechanical, and physical properties. Atomic force microscopy revealed changes in surface roughness due to the incorporation of RT serum into the rubber matrix. FTIR spectroscopy confirmed the successful incorporation of RT serum into the patch, with no chemical interactions observed. The peel and shear strength of the RT patch were 1.46 × 10 −2 N/m and 3.76 × 10 4 N/m 2 , respectively. RT patches had a water contact angle of 109°, indicating hydrophobic surfaces. The dry patch could absorb up to 900 times its weight in solution and release rhodomyrtone, the major bioactive component of RT extract. The amounts of rhodomyrtone released at 2, 8, and 12 h were 0.241 ± 0.001, 0.246 ± 0.001, and 0.202 ± 0.001 mg/L, respectively. Antibacterial activity of RT patches was evaluated using an ex vivo porcine skin infection model. The developed patches demonstrated potent antibacterial activity by inhibiting S. aureus and S. epidermidis by more than 99.9%, compared to blank patches. RT patches exhibited significant scavenging activity and showed no cytotoxic effects on L929 fibroblast cells. In addition, both RT serum and RT patch suppressed the inflammatory process by reducing nitric oxide production in macrophage cells, resulting in decreases of up to 80% and 60%, respectively. The findings highlight the promising potential of the RT patch as an effective alternative for the treatment of acne vulgaris.
Chompraman et al. (Thu,) studied this question.