INTRODUCTION: Acellular dermal matrices are increasingly applied in regenerative medicine and wound healing because they mimic native dermal structure and exhibit low immunogenicity. However, decellularization methods vary, and no consensus exists on the optimal protocol for human full-thickness dermis. This study compared three decellularization approaches to identify the most effective method for generating biocompatible, structurally preserved acellular dermis suitable for clinical use. METHODS: wound healing was evaluated in a murine full-thickness wound model. RESULTS: . Neoangiogenesis and graft orientation showed minimal influence on healing. CONCLUSION: Triton X-100-based and freeze-thaw/surfactant protocols achieved optimal balance between cell removal and matrix preservation, offering promising strategies for producing clinically applicable acellular dermal scaffolds.
Mahdipour et al. (Mon,) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: