Background: Next‑generation nicotine delivery systems, including electronic cigarettes (E‑cig) and heated tobacco products (HTP), are often promoted as reduced‑risk alternatives to conventional tobacco (CT). However, the extent to which they limit user exposure to harmful chemicals remains insufficiently characterized 1. Objective: In this study, we compared the volatile organic compounds (VOCs) present in native materials (pre-heating) with those emitted during the heating or combustion of E-cigs, HTPs, and CTs. Methods: For each product category, the three most commercially popular brands in Portugal were selected. Native matrices (138 ± 3.84 mg of tobacco or e‑liquid) were sealed in glass vials for analysis, while aerosols/smoke were produced under controlled puffing or smoking conditions using standardized machine‑smoking protocols 2,3 and collected in dedicated flasks. VOCs from native materials and aerosol/smoke were extracted via headspace solid‑phase microextraction (HS‑SPME) and dichloromethane solvent extraction, followed by gas chromatography–mass spectrometry (GC–MS). Results: More than 100 compounds were identified across all products. The volatile profiles of unheated HTP sticks and CT shared notable similarities, with ketones, alcohols, terpenoids, and pyridine derivatives dominating. In contrast, e‑liquids displayed more chemically diverse signatures enriched in alcohols, esters, pyranones, and lactones. Thermal processing substantially reshaped these profiles, generating VOCs which were absent from the native materials. HTP aerosols showed additional aldehydes and ketones, E‑cig aerosols contained newly esters, and CT combustion produced benzenoid compounds and polycyclic aromatic hydrocarbons, which were not detected in E‑cig or HTP aerosols. Conclusion: Importantly, many VOCs detected in E‑cig and HTP aerosols currently lack hazard classification under the Globally Harmonized System (GHS), underscoring major gaps in toxicological knowledge. Overall, these findings demonstrate that thermal processes induce extensive chemical transformations across all product types, highlighting the need for comprehensive toxicological evaluation of both known and uncharacterized constituents to support evidence-based regulatory policies.
Monteiro et al. (Wed,) studied this question.