SQ-KFP: A Framework for Spatially Quantitative Metabolic Flux Analysis Enables Imaging the In Vivo Absolute Metabolic Enzymatic Reaction Rate

Key Points

• The aim is to develop a framework for quantitative imaging of metabolic reaction rates in vivo.
• Developed the SQ-KFP framework for spatially quantitative metabolic flux analysis.
• Utilized various detection methods to validate the results.
• Analyzed metabolic flux across different tissues and organisms.
• Established that metabolite concentrations cannot replace direct measurements of reaction rates.
• Provided quantitative imaging that offers insights into metabolic processes across various tissues.