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Ascorbate (AsA) peroxidase can be inactivated both by p-chloromercuribenzoate and by the depletion of AsA but guaiacol peroxidases, such as horseradish peroxidase, cannot. The cytosolic isozymes of AsA peroxidase are less sensitive to depletion of AsA than the chloroplastic isozymes, which include stromal Chen and Asada (1989) Plant Cell Physiol. 30: 987 and thyla-koid-bound Miyake and Asada (1992) Plant Cell Physiol. 33: 541 enzymes. Exploring these properties, we established simple methods for separate assays of AsA peroxidase and guaiacol peroxidase and of the three isozymes of AsA peroxidase in plant extracts. These methods were used to characterize the guaiacol peroxidases and isozymes of AsA peroxidase in plants and algae.
Amako et al. (Sat,) studied this question.