Abstract Introduction Premature ovarian failure is one of the most prevalent conditions among women of reproductive age with fertility problems. The aim of our study was to find out whether intraovarian injection of platelet-rich plasma (PRP) reactivated folliculogenesis in an ovine animal model. Materials and Methods we used 4 sheep that received intraovarian injections of autologous PRP into the right ovary via laparoscopic surgery, while the left ovary served as a control. Three months later, a robot-assisted laparoscopic oophorectomy was performed, and the ovarian tissue was analysed for the expression of end-product genes of the Hippo signalling pathway, which is involved in follicular reactivation. Blood samples were collected monthly to measure the concentration of hormones involved in folliculogenesis and ovarian reserve (AMH, FSH, progesterone, and oestradiol) using ELISA. Results Genetic analysis showed an increase in the expression of the genes CCN2, CCN3, CCN5, CCN6 (growth factors), and BIRC1 (anti-apoptotic). An increases in AMH (2.86 ng/mL) and FSH (1.50 ng/mL) levels was observed one month after the intervention, followed by a subsequent decrease. Oestradiol levels dropped during the first month after the intervention (83.69 pg/mL) and remained low; whereas progesterone levels decreased (8.72 ng/mL) one month post-intervention, but later increased, returning to baseline levels (10.98 ng/mL). Conclusions Intraovarian PRP injection activates the Hippo signalling pathway in the short term, as well as AMH production, but further studies are needed to confirm the folliculogenesis reactivation.
Martínez-Rodríguez et al. (Fri,) studied this question.