Dual-Parameter Self-Calibrating Probes for Organelle Polarity by Decoupling Viscosity from Polarity

Key Points

• This research aims to develop a new type of fluorophore that can measure cell polarity without being affected by viscosity.
• Developed a novel fluorophore using steric hindrance to resist viscosity interference.
• Evaluated fluorophore performance in various cellular environments.
• Compared results with traditional polarity sensing methods.
• Demonstrated enhanced polarity sensitivity with the new fluorophore.
• Resisted viscosity interference effectively, improving measurement accuracy.