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In Paper I (1) methods were described for the isolation of dideoxyribonucleotides from digests of deoxyribonucleic acid (DNA) by pancreatic deoxyribonuclease (DNase). Dinucleotides composed of unlike mononucleotides were obtained as mixtures of the two possible sequential isomers. Methods have now been developed for the analysis of the proportions of the isomers in such mixtures and applied to the dinucleotide fractions obtained by the action of DNase on DNA from calf thymus.
Robert L. Sinsheimer (Mon,) studied this question.