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July 1, 1994Circulation

Acute myocarditis. Rapid diagnosis by PCR in children.

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Why the study?

Does PCR analysis of myocardial tissue improve the detection of viral genomes in children with suspected acute myocarditis?

Population

34 children with suspected acute viral myocarditis and 17 control patients with congenital heart disease or…

Comparison

Polymerase chain reaction analysis of myocardial… vs Control patients without myocarditis; comparison…

Design

Case-control

Key result

PCR detected viral genomes in 68% of myocardial samples from children with suspected acute myocarditis, whereas all control samples were negative.

Authors

AMAmeeta B. MartinSWSteve WebberFFF. Jay Fricker

Discussion

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Overview

Myocardial PCR may aid etiologic diagnosis in suspected pediatric myocarditis; leaves open effects on management.

Study Design

Type

Case-Control (n=51)

Structured PICO

Does PCR analysis of myocardial tissue improve the detection of viral genomes in children with suspected acute myocarditis?

P
Population
34 children with suspected acute viral myocarditis and 17 control patients with congenital heart disease (n=14) or hypertrophic cardiomyopathy (n=3)
I
Intervention
Polymerase chain reaction (PCR) analysis of myocardial tissue samples for enterovirus, cytomegalovirus (CMV), adenovirus, and herpes simplex virus (HSV)
C
Comparator
Control patients without myocarditis; comparison with standard histopathology and viral cultures
O
Outcome
Detection of viral genome in myocardial tissue samplessurrogate

Main Result

Absolute Event Rate: 68% vs 0%

PCR is a rapid and sensitive diagnostic method that enhances the detection of viral genomes, particularly adenovirus and enterovirus, in the myocardium of children with suspected acute myocarditis.

Cite This Study

Martin et al. (1994) conducted a case-control in Acute viral myocarditis (n=51). Polymerase chain reaction (PCR) vs. Control patients was evaluated on Detection of viral genome in myocardial samples. PCR detected viral genomes in 68% of myocardial samples from children with suspected acute myocarditis, whereas all control samples were negative.

synapsesocial.com/papers/6a0f0e279df4132b62f9e3f1https://doi.org/10.1161/01.cir.90.1.330
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