Vessel dilator, long-acting natriuretic peptide, kaliuretic peptide, and atrial natriuretic peptide decreased human pancreatic adenocarcinoma cell numbers by 65%, 47%, 37%, and 34% within 24 hours.
Do four peptide hormones (long-acting natriuretic peptide, vessel dilator, kaliuretic peptide, and atrial natriuretic peptide) decrease the number and proliferation of human pancreatic adenocarcinoma cells in culture?
Four peptide hormones, including atrial natriuretic peptide, significantly decrease the number and proliferation of human pancreatic adenocarcinoma cells in vitro via inhibition of DNA synthesis.
p-value: p=<0.001
BACKGROUND: Long-acting natriuretic peptide, vessel dilator, kaliuretic peptide and atrial natriuretic peptide are four peptide hormones synthesized by the same gene. Their main known biologic properties are sodium and water excretion and blood pressure lowering in both animals and humans. METHODS AND MATERIALS: These four peptide hormones, each at their 1-microm concentrations, were evaluated for their ability to decrease the number and/or proliferation of human pancreatic adenocarcinoma cells in culture at 24, 48, 72 and 96 h. RESULTS: Vessel dilator, long-acting natriuretic peptide, kaliuretic peptide and atrial natriuretic peptide decreased the number of human pancreatic adenocarcinoma cells in culture by 65% (P<0.001), 47% (P<0.01), 37% (P<0.05) and 34% (P<0.05), respectively, within 24 h. This decrease was sustained without any proliferation of the cancer cells occurring in the 3 days following this decrease in number. The mechanism of these peptide hormones' decrease in cancer cell number and antiproliferative effects was a 83% (P<0.001) or greater inhibition of DNA synthesis but not owing to enhanced apoptosis, i.e. programmed cell death. The two known mediators of these peptide hormones' mechanism(s) of action, i.e. cyclic GMP and prostaglandin E2, inhibited DNA synthesis in these adenocarcinoma cells by 51% and 23%, respectively. CONCLUSIONS: Four peptide hormones significantly decrease the number of pancreatic adenocarcinoma cells within 24 h and inhibit the proliferation of these cells for at least 96 h. Their mechanism of doing so is via inhibition of DNA synthesis mediated in part by cyclic GMP.
Vesely et al. (Tue,) conducted a other in Human pancreatic adenocarcinoma cells. Four peptide hormones (vessel dilator, long-acting natriuretic peptide, kaliuretic peptide, atrial natriuretic peptide) was evaluated on Decrease in the number of human pancreatic adenocarcinoma cells in culture within 24 h (p=<0.001). Vessel dilator, long-acting natriuretic peptide, kaliuretic peptide, and atrial natriuretic peptide decreased human pancreatic adenocarcinoma cell numbers by 65%, 47%, 37%, and 34% within 24 hours.