Gene Expression During Normal and Malignant Differentiation

Book reviews on recombinant DNA methodology.This one is based on a practical course run by the University of Leicester and contains five detailed articles: Mammalian cDNA cloning; Analysis of nucleic acids (restriction maps, Southern and Northern blots); Systems for the analysis of gene expression; Cloning in phage lambda; and electron microscopy of nucleic acids.Thus, it covers some areas that are not dealt with in other widely used texts such as that of Maniatis et al.The strength of this book is the large amount of practical detail that is included, especially useful for beginners in this field.Its coverage is limited to the five topics listed above and would therefore need to be supplemented by other articles.However, no attempt is made to guide the reader to any of this material, and in general the references given are rather few in number and not particularly up to date.For example, in the chapter on lambda cloning there is no mention of the recently developed and extremely useful EMBL series of vectors.