Functional network topography of the medial entorhinal cortex

Significance The investigation of the topographic organization of spatially coding cell types in the medial entorhinal cortex (MEC) has so far been held back by the lack of appropriate tools that enable the precise recording of both the anatomical location and activity of large populations of cells while animals forage in open environments. In this study, we use the newest generation of head-mounted, miniaturized two-photon microscopes to image grid, head-direction, border, as well as object-vector cells in MEC and neighboring parasubiculum within the same animals. The majority of cell types were intermingled, but grid and object-vector cells exhibited little overlap. The results have implications for network models of spatial coding.