Bacterial Glycogen Synthesis and Its Regulation

ALLOSTERIC REGULATION OF ADP-GLUCOSE-PYROPHOSPHORYLASE ACTIVITY ... . . . . . . . . . .... .. . . . . . . . . . . . . . . . . . . .. ........ .. . . . . . . . . . . . . . . . . .. .. . . . . . . . . .... 425 Activators. .. . . .. . . . . . . . . . . . .. ... . . . . . . . . . . . . . . . . ...... . . . . . . . . . . . . . . . . . . . . . . . . . .. .. . . . . . . . . . . .. . . . . . 425 Inhibitors ....... 428 EvidenCE' Indicating that Allosteric Regulation of Bacterial ADP-glucose Pyrophosphorylase Has In Vivo Functionality..... . . . . . . . . 432 EVOLUTIONARY RELATIONSHIPS OF THE ADP-GLUCOSE PYROPHOSPHORY­ LASES BASED ON ACTIVATOR SPECIFICITY AND IMMUNOLOGICAL RELATIONSHips. . . . . . . . . . . . . . . . . .... .. . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . .. . . . . . . 432 BIOCHEMICAL STUDIES OF THE ACTIVATOR BINDING SITE OF E. COLI ADPGLUCOSE PYROPHOSPHORYLASE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 437 Specificity of Activation . . . . . . . ...... . . . . . . . . . . ..... . . . . . . . . . ...... .... . . . . . . . . . . .... 437 Chemical Modification of the Activator Site of the E. coliADP-glucose Pyrophosphorylase . . .... ......... 438 Compar/son of the Amino-Terminal Sequences of Other Bacterial ADP-glucose Pyrophosphorylases with the E. coli ADP-glucose Pyrophosphorylase ... 440 GENETIC REGULATION OF GLYCOGEN SYNTHESIS IN E. COLI AND IN S. 1YPHIMURIUM . . . ... .. . . . . . . . . . . . . .... . . . . . . . . . . . . . . . . . . . ... . . . . . . . . . . . . . . . . . ... . . . . . . . 442 Possible Regulation by Cyclic AMP .... .. . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . .. . . .. . . . . . . . 443 Location of the Glycogen Genes in the Linkage Maps ofE. coli B. E. coli K12. and S. typhimurium LT-2 444 Cloning of the Glycogen Biosynthetic Enzyme Structural Genes of E. coli . . . . . . . . . . .. 446