Abstract The present study aimed to establish a robust propagation protocol for the endangered medicinal species Aquilegia fragrans through rhizome cuttings and in vitro micropropagation techniques. The effects of various plant growth regulators (PGRs) such as GA 3 , BAP, and IBA on vegetative propagation, including sprouting, root and shoot development, were investigated. Rhizome cuttings treated with 150 ppm GA 3 exhibited the highest sprouting percentage (83.34%) and significant enhancement in growth parameters, including shoot and root length, leaf number, and rhizome development. Additionally, the study assessed the influence of different soil compositions on sprouting success, with a mixture of soil, sand, and vermicompost (1:1:1) yielding the highest sprouting rate and optimal morphological traits. The micropropagation protocol was further optimized by evaluating surface sterilization techniques and the effect of cytokinins, particularly Meta-topolin and TDZ, in combination with auxins (IAA, IBA, and NAA). The combination of TDZ (3.5 mg L −1 ) and IBA (1.25 mg L −1 ) was found to induce the highest regeneration frequency (82.00%), with a maximum of 6 shoots per explant and a reduced induction period. Acclimatization of in vitro -derived plantlets under greenhouse conditions resulted in a 75% survival rate, confirming the feasibility of this propagation method for large-scale cultivation and conservation.
Bhat et al. (Thu,) studied this question.
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