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Different methods of microanalysis (energy of wavelength dispersive X-ray spectrometry microprobes, ion microanalyser) combined with histochemistry, allowed us to analyse the cement of the tube and the main glands connected to the building organ of Sabellaria alveolata (L.). These methods clearly showed a mineral component of which the main elements are phosphorus, probably linked to the organic component, calcium, and magnesium, with some manganese and iron. The biomineral is produced by glandular cells having "heterogeneous spherulae." The quick hardening of this cement probably takes place independent of and before the quinonic tanning of the inner organic lining of the wall of the tube.
Gruet et al. (1987) studied this question.