What type of study is this?

This is a Animal Study study (also classified as: Observational).

August 17, 2025

Novel Papain-Elastase Induced Murine Model for Infrarenal Abdominal Aortic Aneurysm Rupture

Key Points

The murine model showed a striking 93% AAA rupture rate when treated with angiotensin II, exemplifying its potential to mimic human pathology.
Mice receiving both papain and porcine pancreatic elastase displayed a significant increase in aortic diameter and inflammation markers within two weeks post-induction.
Introduction of β-aminopropionitrile led to a staggering 500% growth in chronic AAAs, highlighting its impact on aneurysm progression.
These findings suggest that the new models can enhance research in AAA therapy and understanding of its pathophysiology in humans.

Abstract

Abstract Abdominal aortic aneurysm (AAA) rupture leads to high morbidity and mortality. Current rodent models struggle to reliably mimic infrarenal AAA rupture. Chemical treatments using porcine pancreatic elastase (PPE), papain (Pap), β-aminopropionitrile (BAPN), and angiotensin II (ANG II) are known to induce AAA in rodents. We hypothesized that combining these agents could establish reliable chronic AAA and acute rupture models, resembling human pathology. Here AAAs were induced in male C57BL/6 mice using peri-adventitial exposure to PPE, Pap, or a combination (PPE + Pap), with or without 0.3% BAPN and ANG II. Two weeks post-induction, Pap and PPE + Pap showed increased aortic diameters, higher inflammation, elastase degradation, and matrix metallopeptidase (MMP) activity. Addition of BAPN resulted in large chronic AAAs (500% growth) and intraluminal thrombus (ILT) formation. ANG II-treated mice exhibited a 93% rupture rate, increased inflammation, MMP activation, and ILT formation. These novel murine models are ideal for investigating AAA pathophysiology and therapeutic discovery.

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