Herbal plants have long been used in traditional medicine to address and heal a wide range of health-related issues. Withania coagulans (Family; Solanaceae), a valuable medicinal plant used to evaluates bioactives by using maceration. Phytochemical analysis evaluated by qualitatively for five different extract. Methanol extract has highest phytocostitutents so further used for analysis phenolic compounds (86.67 ± 3.19 mg GAE/g) and flavonoids (60.95 ± 2.70 mg QE/g). The methanol extract demonstrated significant antioxidant activity in the DPPH and FRAP assay with IC50 value 63.02±1.08 μg/ml and 70.2±1.11 μg/ml respectively and notable antibacterial activity against Escherichia coli and Staphylococcus aureus. Additionally, the methanol extract exhibited substantial anti-diabetic potential, with IC50 value 602.6±2.98 μg/ml. This study demonstrated that the methanolic extract of Withania coagulans is a potent natural antioxidant, which could be beneficial in slowing the progression of various oxidative stress-related conditions
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