Figure 2 from HuR-Regulated Extracellular Vesicles Promote Endothelial Cell Remodeling in Pancreatic Cancer

Endothelial cell abundance correlates with ELAVL1 expression, and endothelial cells import PDAC EVs. A, Correlation between ELAVL1 expression in PDAC cells and the presence of endothelial cells in publicly available patient single-cell RNA-seq. B, Within this cohort, a comparison of the endothelial cell percentage in patients with the highest and lowest quartiles of ELAVL1 expression. C, Representative images of HUVECs and HPaMECs treated with PKH67-labeled PANC-1 WT vs. HuR-KO EVs for 4 hours with stained nuclei (blue, DAPI) and cytoskeleton (phalloidin, red; scale bar, 50 µm). D, Relative cell area and levels of EV import pixels (px)/cell area of HUVECs and (E) HPaMECs after 4-hour EV treatment (n = 3). P values were calculated using an unpaired two-tailed Student t test. *, P P P