Abstract The development of Sulphonamides is a fascinating and informative area in medicinal chemistry, its functional group has a long and rich history in organic chemistry and drug discovery. The objective of this work is to synthesize and characterize 4-methylbenzenesulphonamides derivatives using 4-methylbenzenesulphonyl chlorides and amino acids (leucine, histidine, phenylalanine, and cysteine) as precursors, then, biological studies were carried out. The FTIR spectroscopic results confirm characteristic functional group, p-disubstituted benzene, -SO2-NH2, R3N, C=O, R2NH, Amide C=O, the IH-NMR spectrum, the peaks confirm 2o amine, p- disubstituted benzene), m- disubstituted benzene), CH3-n and CH3-CO and the 13CNMR, (acetyl C=O), (C-S=O), amide C=O, (C-H), acetyl CH3, -CH3, aromatic carbons); the results of the in silico antibacterial studies disclosed that the range of the affinity of binding is between -6.3 to -8.7 kcal/mol, with the ligands interacting more positively than other investigated microbes with the staphylococcus variant's 6xg5 receptor, while the results of the in vitro antibacterials studies showed that at 200 mg/mL, the test organisms exhibit a zone of clearance or inhibition that varies in size from 0 to 28 mm. The spectroscopic results support the proposed structures of the compounds, the synthesized compounds have significant antibacterial potency in the respective bacteria cells, as demonstrated by the in silico antibacterial studies; these findings suggest that the synthesized compounds could be used as future antibacterial agents. According to the in vitro antimicrobial investigation, the majority of the produced compounds had antibacterial properties.
David et al. (Thu,) studied this question.