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September 12, 2025

A REVIEW ARTICLE ON DNA BARCODING AND MOLECULAR APPROACH FOR IDENTIFICATION OF Labeo bata (Hamilton-1958) BY USING A GENE (CYTOCHROME C OXIDASE1 - CO1) FROM PAKISTAN

Key Points

DNA barcoding effectively identifies unknown species using the cytochrome c oxidase1 gene, interpreting genetic information for classification.
The study highlights the significance of labeo bata as an important food species in Pakistan, emphasizing its relevance to local fisheries.
PCR products were analyzed with bioinformatic tools to establish phylogenetic relationships among different labeo species.
The approach serves as a milestone in molecular identification, demonstrating the utility of dna barcoding in biodiversity studies.

Abstract

DNA barcoding is a new perspective that uses gene sequence Cytochrome C oxidase subunit I (COI) for identification of unknown species by using "barcodes". The present study of” Labeo bata” specie was conducted for identification because it is important food specie in Pakistan. PCR products were analyized by bioinformatic tools. Evolutionary tree based on kimura 2 parameter indicate phylogenetic relationship among labeo species.A specimen is recognized if the barcode sequence of unknown specimen resembles the sequence in the barcode library or else, a new record is determined. It was concluded that DNA barcoding serves as milstone for identification at molecular level.

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