The following protocol describes the preparation of urine samples for non-targeted analysis by mass spectrometry coupled to liquid chromatography as proposed by the PFEM (MetaboHUB-Clermont). A pre-analytical normalisation step is carried out based on the osmolality measurement determined for each sample. The steps cover the sample preparation and quality control. The expected outcome of this protocol is a list of urine samples ready for analysis by LCMS.
Joly et al. (Fri,) studied this question.