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L-DOPA, or levodopa, plays an important role in the treatment of Parkinson's disease, a debilitating neurological disorder. It acts as a precursor to dopamine, a neurotransmitter crucial for the regulation of motor functions. Administered orally, L-DOPA easily crosses the blood-brain barrier and converts into dopamine in the brain, relieving symptoms such as tremors and rigidity. However, its prolonged use can lead to complications. A significant concern with L-DOPA is its conversion to dopaquinone, a quinone metabolite that enters the redox cycle and continuously produces hydrogen peroxide. In addition, L-DOPA, which resembles tyrosine with an additional hydroxyl group, can randomly incorporate into the proteins of dopaminergic neurons and thus become an additional source of oxidative stress in Parkinson's patients. In this study, we scrutinized the rate-limiting step of L-DOPA autoxidation in aqueous solution. The reaction we studied is an intramolecular Michael addition concerted with a proton transfer from the amino group. Using the Empirical Valence Bond (EVB) method, we computed the free energy profiles of the reaction in water. The calculated barrier of 30.93 ± 1.12 kcal/mol is in reasonable agreement with the experimental barrier of 27.55 kcal/mol. This agreement confirms the validity of the studied mechanism and demonstrates the applicability of our simulation methodology for studying the autoxidation kinetics of L-DOPA within proteins.
Prah et al. (Sat,) studied this question.