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Abstract: Western Ghats is one of the major tropical evergreen-forested regions in India and is one of the hottest hot spots in the World. Increasing urbanization as well as deforestation and thereby resulting habitat loss leads us to give a hand on conservation of such challenging biodiversity in these region. Aerides crispa and Cleisostoma tenuifolium are two vulnerable orchid plants from Kodagu, which is the heart of Western Ghats and are used as source of endophyte for the study. Isolation and molecular characterization of endophytic bacteria from the plants were studied to its genotypic level by comparing the PCR isolated 16S rRNA sequences. Nucleotide sequences were compared with the NCBI database and accession numbers for each bacterial strain were obtained from the GenBank. About 12 different bacteria were molecularly identified from these two orchids, A. crispa and C. tenuifolium.
S Darsha (Wed,) studied this question.