Figure 1 from Generation, Transcriptomic States, and Clinical Relevance of CX3CR1+ CD8 T Cells in Melanoma

Frequency, transcriptomic phenotype, and clinical relevance of CX3CR1+ CD8+ T cells in human melanomas. A, Representative histogram showing CX3CR1 expression of CD8+ T cells in human melanomas (blue). Isotype-matched controls are shown in red. Gating strategy is shown in Supplementary Fig. S2. The right shows frequency of CX3CR1+ subset in CD8+ T cells (n = 7). B–F, Single-cell profiling of melanoma-infiltrating T and NK cells from patients treated with ICI therapy in the Sade-Feldman et al. data. Final cell-type annotations of total cells and subsequent filtering of T and NK- cells are shown in Supplementary Fig. S4A and S4B. B, UMAP plots of melanoma-infiltrating T and NK cells from responders and nonresponders before and after ICI therapy. C, Expression patterns of indicated genes in T- and NK-cell clusters in melanoma. Expression levels are color-coded: gray, not expressed; purple, expressed. D, Heatmap displaying the top 10 significantly enriched genes found in each cluster. E, Violin plots show expression of PDCD1, TIGIT, and HAVCR2 in CX3CR1+ and CX3CR1− populations. Differential expression is determined by Wilcoxon rank sum test. F, Proportions of cell clusters observed in individual melanomas from responders (R) and nonresponders (NR) at pretreatment (Pre) and post-treatment (Post).