Quantitative indicators of peripheral blood of rats when studying the chronic toxicity of the chelate-mineral complex in the form of an aqueous solution for injection

The work describes the effect of a chelate-mineral complex in the form of an aqueous solution for injection with repeated administration on survival, clinical condition, dynamics of body weight gain and changes in the concentration of formed elements in the peripheral blood of laboratory rats on the 7th, 14th, 21st and 28th days. e day from the start of drug administration as part of a chronic toxicity study. To conduct the experiment, 3 groups of male laboratory rats, 10 animals each, were formed. A therapeutic dose (0.05 ml/100 grams) and a dose between LD50 and therapeutic (0.47 ml/100 grams) were selected as study doses. The solution was administered intramuscularly, once a day, for 30 days. During the studies, it was established that the drug at a dose of 0.05 ml/100 grams with long-term administration did not cause death or changes in the clinical condition of laboratory animals, and had a positive effect on the average daily gain in live weight. An increase in the number of red blood cells and hemoglobin was observed in the blood from day 7. With repeated administration of the chelate-mineral complex in the form of an aqueous solution for injection at a dose of 0.47 ml/kg, no cases of death were recorded; during a clinical examination from days 15 to 20, diarrhea was observed without other visible changes in the general condition. The average daily increase in live weight over 30 days was on average 56.1% lower than in rats receiving a therapeutic dose. In the blood - leukocytosis and an increase in the number of red blood cells, starting from 7 days after the start of the experiment.