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Introduction 0.05), and 55 proteins significantly downregulated, including Insr (Log2FC ≤ -1, Adj. PV0.05). The application of Principal Component Analysis to our proteomic data revealed a tightly clustered pattern that segregated according to glucose exposure. Our analysis revealed distinct regulatory patterns in cellular pathways in response to 72 h sustained high glucose. Among the prominent upregulated pathways were protein N-linked glycosylation, the ubiquitin-dependent ERAD pathway, and protein biosynthetic processes. Conversely, downregulated pathways predominantly centered around metabolic regulation and mitochondrial processes, including various components of the oxidative stress response and mitochondrial electron transport chain. Conclusion: Considering the importance of glucose adaptation of islet function to the preservation of glucose homeostasis, this study provides a glucose response proteomic signature that raises known and novel targets for further exploration. Disclosure M. Khosravifar: None. M.W. Haemmerle: None. S. Sen: None. D.A. Stoffers: Other Relationship; Eiger BioPharmaceuticals. Funding National Institutes of Health (R01-DK-122039)
KHOSRAVIFAR et al. (Fri,) studied this question.