Can thermal anomalies impair the restoration of Cystoseira s.l. forests?

Marine macroalgal forests are facing unprecedented challenges worldwide due to the accelerating impacts of climate change. These ecosystems play a crucial role in supporting biodiversity, coastal ecosystem functions and services, and are indeed object of several conservation and restoration measures. The Mediterranean Sea is warming faster than the oceans and thermal anomalies are occurring with increasing intensity, frequency and duration. Along the Mediterranean coasts, Cystoseira sensu lato species are the main representatives of macroalgal forests and their decline has been widely documented. Some relevant achievements in the implementation of ecological restoration have been obtained, but rising temperatures and the occurrence of thermal anomalies increasingly threaten the success of these restoration attempts. In the summer of 2022, ex-situ restoration actions of Ericaria amentacea were carried out by collecting fertile material from three donor sites of the Italian coasts along a latitudinal gradient, during the period of sexual maturity (June/July). Noteworthy during the summer of 2022, anomalous thermal conditions were recorded at the donor sites, with sea surface temperatures exceeding the climatological mean up to 4.3°C and heatwaves lasting up to 78 days. Our results suggest that these thermal anomalies may have affected the culture of the embryos in both the pre- and post-zygotic phases, resulting in significantly low culture efficiency at the three donor sites. The reproductive structures showed some abnormalities, fertilization of eggs was lower and embryo growth was slower, resulting in lower percent cover of seedlings on the tiles and lower survival rate. The observations underscore the vulnerability of Mediterranean algal forests to global change and highlight additional challenges for their restoration due to the increasing frequency and severity of thermal anomalies, emphasizing the need for adaptive strategies and a comprehensive understanding of the species in a changing climate. Marine forest restoration requires long lasting projects, to allow for long-term monitoring and better understanding the biology of the species and for mitigating stochastic events that can cause the temporary failure of efforts.