Pd03-09 BPH Patients With Metabolic Syndrome Exhibit Specific Increases in Gene Expression Relating to Inflammation and Immune Reactivity

You have accessJournal of UrologyBenign Prostatic Hyperplasia: Basic Research & Pathophysiology (PD03)1 May 2024PD03-09 BPH PATIENTS WITH METABOLIC SYNDROME EXHIBIT SPECIFIC INCREASES IN GENE EXPRESSION RELATING TO INFLAMMATION AND IMMUNE REACTIVITY Matthew N. Simmons, Amritha N. Sreekumar, Martha N. Terris, Pablo Santamaria, Huidong N. Shi, and Sharanjot N. Saini Matthew N. SimmonsMatthew N. Simmons , Amritha N. SreekumarAmritha N. Sreekumar , Martha N. TerrisMartha N. Terris , Pablo SantamariaPablo Santamaria , Huidong N. ShiHuidong N. Shi , and Sharanjot N. SainiSharanjot N. Saini View All Author Informationhttps://doi.org/10.1097/01.JU.0001009388.01015.e5.09AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Molecular studies support the presence of two molecular subtypes of BPH, namely BPH-A which exhibits stromal signature gene expression and BPH-B which is associated with metabolic derangements and presence obesity and hypertension (Liu et al. Nature Commun 2020 11(1): 1987). This study directly compared gene expression in BPH patients with and without metabolic syndrome. METHODS: Patients with severe BPH undergoing TURP were assessed. A MetS score (MetX) was assigned based on points assigned for BMI severity and presence of hypertension, hyperlipidemia and type 2 diabetes mellitus. Eleven samples from BPH patients without MetS and 8 samples from BPH patients with MetS were analyzed. Tissues were acquired immediately during bipolar TURP and placed directly in RNA preservation solution. Tissue preps were conducted with RNeasy kits (Qiagen). Nanodrop and formal QC analysis was conducted. Gene expression was profiled using the Clariom S™ assay (Applied Biosystems). Array data was normalized and high data quality indicated by an AUC of ≥0.948 was observed for all samples. Gene set enrichment analysis (GSEA) was conducted. Significant associations were identified by false discovery rate (FDR) corrected p-values (q value) of ≥0.25. RESULTS: GSEA showed that BPH+MetS tissues exhibited specific and increased expression of multiple immune-related gene sets including adaptive immune response, B-cell and lymphocyte-mediated immunity, antigen receptor-mediated signaling, macrophage activation, Toll-like receptor signaling and others. Hallmark gene sets identified included those for interferon gamma response, IL6-Jak/Stat3 signaling and interferon alpha response. CONCLUSIONS: Transcriptomic analysis implies a significant immune component in BPH patients with metabolic syndrome. This may represents a distinct disease entity or it is possible that MetS-associated immune changes accelerate a common disease process. Studies are ongoing to identify key immune processes involved in BPH progression and association with LUTS. Source of Funding: Medical College of Georgia © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e79 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Matthew N. Simmons More articles by this author Amritha N. Sreekumar More articles by this author Martha N. Terris More articles by this author Pablo Santamaria More articles by this author Huidong N. Shi More articles by this author Sharanjot N. Saini More articles by this author Expand All Advertisement PDF downloadLoading ...