Mp69-15 Metagenomic Sequencing of the Urinary Microbiome of Postmenopausal Women With Recurrent Urinary Tract Infection and Type 2 Diabetes

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder I (MP69)1 May 2024MP69-15 METAGENOMIC SEQUENCING OF THE URINARY MICROBIOME OF POSTMENOPAUSAL WOMEN WITH RECURRENT URINARY TRACT INFECTION AND TYPE 2 DIABETES Sara B. Papp, Michael L. Neugent, Belle M. Sharon, Philippe E. Zimmern, and Nicole J. De Nisco Sara B. PappSara B. Papp , Michael L. NeugentMichael L. Neugent , Belle M. SharonBelle M. Sharon , Philippe E. ZimmernPhilippe E. Zimmern , and Nicole J. De NiscoNicole J. De Nisco View All Author Informationhttps://doi.org/10.1097/01.JU.0001008892.86171.de.15AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Recurrent urinary tract infections (rUTI) are common in postmenopausal women with type 2 diabetes mellitus (T2DM). However, little is known about the composition of the urinary microbiome in this population and how it contributes to rUTI susceptibility. We aimed to identify key differences in urinary microbiome composition and function between women with and without T2DM through shotgun metagenomic sequencing of their urine. METHODS: Following IRB approval, urine samples were collected from postmenopausal T2DM women across 3 groups: rUTI remission (rUTI history), rUTI relapse (active infection), and no history of UTI. Groups were age and estrogen-matched and DNA was purified from 20 mL of urine per patient. Sample concentrations were standardized and 20 pg of spike-in DNA of a rare bacterial genome was added. Shotgun metagenomic sequencing was performed (Illumina NextSeq2000), reads were quality controlled and purified of human-mapping sequences (CLC Genomics Workbench), and all microbial reads were taxonomically analyzed (MetaPhlan3). For further controls, data for non-T2DM women in the same groups were obtained. RESULTS: Fifteen TD2M and 15 non-T2DM samples were compared for this study (Figure 1). The mean genomic DNA yield was 18.5 ng/uL. An average of 75.8% human mapping reads was observed and further censored. The remaining dataset had an average of 5.1 million microbial reads. In the No UTI group, common urogenital flora microbes dominated across all samples in both T2DM and non-T2DM groups. In the rUTI Remission group, urogenital flora microbes were the most common in non-T2DM, while common UTI pathogens were present in T2DM samples. For the rUTI Relapse group, both T2DM and non-T2DM were infected with common UTI pathogens, however, 5/5 diabetic samples showed multi-strain infections while only 2/5 non-diabetic samples did. CONCLUSIONS: We aim to identify differences in urinary microbiomes between women with and without T2DM and various histories of rUTI. Our pilot study suggest that T2DM women may have higher rates of multi-strain infections compared to their non-T2DM counterparts. An expanded study (n=60) is currently underway to study the urinary microbiomes of women with and without T2DM. Download PPT Source of Funding: N/A © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1124 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Sara B. Papp More articles by this author Michael L. Neugent More articles by this author Belle M. Sharon More articles by this author Philippe E. Zimmern More articles by this author Nicole J. De Nisco More articles by this author Expand All Advertisement PDF downloadLoading ...