Virtual Simulation Experiments (VSE) are increasingly applied in medical education, yet their role in medical microbiology remains underexplored. This study evaluated a VSE system designed for the isolation, culture, and identification of enteric pathogens, framed by Self-Determination Theory (SDT) and the Unified Theory of Acceptance and Use of Technology 2 (UTAUT2). Ninety medical students were randomly assigned to either a VSE group or a traditional teaching group. Outcomes were assessed through operational skill and knowledge tests, supplemented by qualitative feedback. The VSE group achieved higher scores in operational skills (89.6 ± 5.2 vs. 81.8 ± 6.3, p < 0.01) and knowledge retention (87.4 ± 4.4 vs. 81.8 ± 5.0, p < 0.01). Students reported that VSE' immersive environment and instant feedback enhanced satisfaction and autonomy, aligning with SDT. VSE significantly improved learning outcomes in medical microbiology and promoted intrinsic motivation. These findings provide empirical support for integrating VSE into medical curricula and highlight the need for further research on its long-term and broader educational impact.
Li et al. (Tue,) studied this question.