ABSTRACT Introduction In a prospective cohort from the Tampa Bay region (2016–2020), patients with autoimmune cytopenia (AIC) were evaluated to identify cellular and serum biomarkers that distinguish those with underlying inborn errors of immunity (IEI). Methods Clinical phenotype and genetic causes of IEI were assessed using targeted panel‐based sequencing. Unique lymphocyte subsets, including activated naïve and transitional B cells, CD19 hi CD21 lo B cells, follicular helper T (T FH ) cells, regulatory T (T reg ) cells, and TCRαβ + CD4 − CD8 − double‐negative T cells (DNTαβ), were assessed by flow cytometry. Serum levels of lipopolysaccharide (LPS), B‐cell activating factor (BAFF), and soluble IL‐2 receptor (sIL2R) were quantified by ELISA. Results Among 104 AIC patients, 53 (51%) showed evidence of IEI, including 27 (26%) with monogenic disorders—most commonly partial DiGeorge syndrome (pDGS), followed by variants in NFKB1, CTLA4, and FAS. The prevalence of IEI was highest in autoimmune hemolytic anemia (AIHA) (62.5%) and Evans syndrome (61.5%). Low levels of IgG, IgA, and IgM, as well as reduced percentages of naïve CD4 + and CD8 + T cells, were significantly associated with increased odds of IEI. In AIC‐IEI patients, transitional B cells, CD19 hi CD21 lo B cells, and T FH cells were expanded, accompanied by elevated serum levels of BAFF and sIL2R. Conclusions Quantitative immunoglobulin levels and naïve T cells remain valuable indicators of IEI in AIC. Our findings highlight the diagnostic value of emerging cellular and serum biomarkers in identifying IEI, including dysregulation of early B‐cell subsets (transitional B cells and CD19 hi CD21 lo B cells), expansion of T FH cells, and elevated levels of BAFF and sIL2R.
Gaal et al. (Wed,) studied this question.
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