Objective Mentha Crispa samples collected from Kien Giang province were systematically identified, and the extraction process was carefully optimized to enhance biological activity. This study focused on developing an extraction method with optimal parameters using response surface methodology, aiming to improve the overall quality of the extract while maximizing its polyphenol and flavonoid content. Method Mentha Crispa was extracted using the microwave-assisted method, with factors such as power, time, and ethanol ratio and solvent volume affecting the process. The biological activities were assessed using several methods, including the DPPH assay, ABTS assay, agar disk-diffusion method, and the Minimum Inhibitory Concentration (MIC) test, Egg Albumin Denaturation assay (EAD). Results The content of polyphenols and flavonoids was evaluated to base on the RSM with the optimal parameters from the model (time microwave at 1 min, power of 260 W, solvent-to-raw material ratio of 2 g/40 mL, ethanol concetration of 50%) of 925 (mgGAE).(gDW) −1 and 163.5 (mgQE).(gDW) −1 (R 2 = 0.999 and 0.997 > 0.75). The antioxidant capacity of the extract was found to be high with DPPH and ABTS results of 0.38 and 3.25 mg.mL −1 . Besides, the antibacterial ability of the sample was also recorded at the minimum inhibitory concentration of Escherichia coli of 10 mg.mL −1 and Staphylococcus aureus of 7.5 mg.mL −1 . In addition, the anti-inflammatory ability of the basil extract was recorded as 41.035% at a concentration of 1 mg/mL. Conclusions The study showed that the microwave-assisted extraction method has shown outstanding time-saving ability. The extraction conditions were optimized to ensure high flavonoid and polyphenol content, with good activity. It can be seen that Mentha Crispa extract has great potential for application in the medical field.
Nguyen et al. (Thu,) studied this question.