Introduction: This study aims to characterize the polypeptide expression profiles in omental metastasis of Ovarian Cancer (OC) and explore their potential applications in the treatment of OC metastasis. Methods: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) was employed to analyze the polypeptide profiles of primary OC tissues, omental metastatic tissues, and nor-mal omental tissues from three OC patients. Differentially expressed polypeptides were screened using bioinformatics, and three polypeptides were synthesized to investigate their functional mechanisms in OC. Results: LC-MS/MS analysis revealed 127 differentially expressed polypeptides in omental metastatic tissues compared to primary OC tissues, and 171 differentially expressed polypep-tides compared to normal omental tissues. Notably, a polypeptide derived from alpha-2-mac-roglobulin (pAHSG) was significantly downregulated in omental metastatic tissues compared to both normal omental tissues and primary OC tissues. In vitro experiments demonstrated that pAHSG inhibited the proliferation and invasion of OC cells in a concentration-dependent man-ner. Flow cytometry further indicated an increase in G1 phase cells and a reduction in S phase cells following pAHSG treatment. Pull-down assays combined with mass spectrometry iden-tified 40 proteins interacting with pAHSG, and KEGG pathway analysis revealed their in-volvement in aminoacyl-tRNA biosynthesis and cancer-related pathways. Collectively, these findings suggest that the downregulation of pAHSG is closely associated with omental metas-tasis in OC. Discussion: The polypeptide expression profiles of omental metastatic tissues in OC exhibit significant alterations. The pAHSG effectively suppresses the malignant behaviors of OC cells, providing a novel potential target for the prevention and treatment of OC metastasis. Conclusion: Compared with primary OC tissues and normal omental tissues, the polypeptide expression profiles of omental metastatic tissues exhibit significant alterations. Notably, pA-HSG, whose level is significantly changed in metastatic tissues, can effectively inhibit the malignant behaviors of OC cells, thereby providing a novel potential target for the treatment of OC metastasis.
Chen et al. (Mon,) studied this question.