The research aims to evaluate how omitting mineral oil affects oocyte maturation and embryo development during in vitro maturation (IVM).
Evaluated oocyte maturation rates without mineral oil overlay.
Assessed hormone bioavailability and oxidative stress levels.
Compared blastocyst formation rates between cultures with and without oil overlay.
Omission of mineral oil resulted in higher oocyte maturation rates.
Improved hormone availability and reduced oxidative stress were observed.
Blastocyst formation rates significantly increased in the absence of mineral oil.
Abstract
Omission of mineral oil overlay enhances hormone bioavailability, diminishes oxidative stress, and significantly improves oocyte maturation and blastocyst formation rates in IVM culture.