Studying the evolutionary potential of ancestral aryl sulfatases in the alkaline phosphatase family with droplet microfluidics

Key Points

• The study aims to explore the evolutionary potential and functional dynamics of ancestral aryl sulfatases within the alkaline phosphatase family.
• Utilized droplet microfluidics for experimental analysis
• Characterized dynamics and functional shifts during enzyme evolution
• Assessed the enzymatic functions of ancestral proteins
• Identified significant functional shifts in ancestral aryl sulfatases
• Showed the potential for evolving desired enzymatic properties
• Highlighted the role of microfluidics in studying protein dynamics