Development and Application of a Tetra-ARMS PCR Assay for Detecting Indel Polymorphisms in the Bovine PRNP Gene

Key Points

• The aim is to develop a Tetra-ARMS PCR assay for detecting indel polymorphisms in the bovine PRNP gene.
• Developed a tetra-arms PCR assay targeting the PRNP gene.
• Analyzed insertion/deletion (indel) polymorphisms in the promoter and intron 1 region.
• Evaluated the assay's effectiveness in detecting genetic variations.
• Successfully detected indel polymorphisms in the bovine PRNP gene.
• Identified variations associated with susceptibility to BSE.
• Demonstrated high sensitivity and specificity of the Tetra-ARMS PCR assay.