What does this research mean for the field?

Tomato leaf curl New Delhi virus (ToLCNDV) is the predominant etiological agent of yellow mosaic disease in cucurbits in Karnataka. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

The research aims to detect and characterize begomoviruses linked to yellow mosaic disease in various gourds in Karnataka.

February 17, 2026Open Access

Molecular characterisation of ToLCNDV isolates causing yellow mosaic disease in gourds of Karnataka

Key Points

The research aims to detect and characterize begomoviruses linked to yellow mosaic disease in various gourds in Karnataka.
Field sampling from ridge gourd, bitter gourd, ivy gourd, and bottle gourd in Karnataka
PCR amplification with specific degenerate primers
Sequencing and BLASTn analysis to identify viral isolates
Phylogenetic analysis to evaluate relationships among virus isolates
Confirmed presence of ToLCNDV with expected PCR fragment sizes
Phylogenetic analysis revealed distinct clustering of isolates based on host and region
Isolates showed 96-100% sequence similarity with known ToLCNDV strains
Established ToLCNDV as the main cause of yellow mosaic disease in selected cucurbits

Abstract

Yellow mosaic disease (YMD) is a major constraint in cucurbit production in India. The present study was undertaken to detect and characterise begomoviruses associated with YMD in ridge gourd (Luffa acutangula (L.) Roxb.), bitter gourd (Momordica charantia L.), ivy gourd (Coccinia grandis (L.) Voigt) and bottle gourd (Lagenaria siceraria (Molina) Standl.) from Chikmagalur, Davanagere and Shivamogga districts of Karnataka in the year 2021. Polymerase chain reaction (PCR) amplification using degenerate primers DengA/DengB and PAL1v/PAR1c confirmed the presence of begomovirus, amplifying expected fragments of ~560 bp and ~1.2–1.3 kb, respectively. Sequencing and BLASTn analysis confirm the presence of Tomato leaf curl New Delhi virus (ToLCNDV). Phylogenetic analysis showed clustering of isolates based on host and region. The ridge gourd (MZ664277), bitter gourd (MZ664278) and ivy gourd (MZ664279) isolates grouped closely, while the bottle gourd (MZ664281) formed a distinct cluster. Identity analyses confirmed 96–100 % sequence similarity with known ToLCNDV strains. The study establishes ToLCNDV as the predominant etiological agent of YMD in these cucurbits in Karnataka.

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Cite This Study

Bhavana et al. (Fri,) studied this question.

synapsesocial.com/papers/699405254e9c9e835dfd5f83 https://doi.org/https://doi.org/10.14719/pst.11155

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