Overexpressed ATP-binding cassette (ABC) transporters, such as multidrug resistance protein 1 (MRP1) encoded by ABCC1, are responsible for multidrug resistance in anticancer treatment due to their abilities to prevent drugs from reaching their lethal intracellular concentrations. Similar overexpression of drug efflux pumps is a major contributor to antimicrobial resistance seen in bacteria. We report sequence-selective, molecularly imprinted nanoparticles (MINPs) targeting MRP1 in human cancer cells. These nanoparticles mask different segments of the long, flexible linker connecting NBD1 (nucleotide-binding domain 1) and TMD2 (transmembrane domain 2) of MRP1. Binding of the protein near the inner membrane interface is found to strongly inhibit the function of the efflux pump and sensitize Dox-resistant cancer cells to the drug, reducing its IC50 value by ∼25%. These results illustrate a new strategy for inhibiting intracellular proteins and identifying potential functional linear motifs in unstructured regions of proteins, benefiting from the facile preparation of the MINPs for different peptide sequences, their highly specific binding abilities, and their ability to enter cells.
Ghosh et al. (Tue,) studied this question.