ABSTRACT Background Peanut allergy is a serious form of food allergy that can lead to anaphylactic reactions. Research has shown that the Ara h 2 allergen is immunodominant and associated with fatal systemic reactions. Methods We determined the crystal structure of recombinant Ara h 2.0201 in a complex with the human‐derived PA12P3D08 (D08) IgE Fab fragment at 3.2 Å resolution. In addition, native mass spectrometry was used to study interactions of D08 with Ara h 2 and its peptides. Results The structure revealed that D08 Fab binds to a long loop of Ara h 2.0201, which contains three repeated DPYSPS motifs. The immunocomplex structure illustrated how three copies of D08 Fabs can bind simultaneously to Ara h 2.0201 in close proximity. Native mass spectrometry studies of D08 Fab with Ara h 2.0201 and peptides containing 2–3 motifs demonstrated cross‐linking of Ara h 2.0201 in solution and the propensity of D08 Fab to self‐associate. Motif peptides from Ara h 2.0201 highlighted the importance of proline hydroxylation for binding affinity. D08 Fab also bound to hydroxyproline‐containing peptides from Ara h 1 and 3. Conclusions The trivalent binding is effective in forming large allergen‐IgE complexes on mast cell or basophil surfaces and contributes to the potency of Ara h 2 in triggering allergic reactions and highlighting its role in anaphylaxis. Proline hydroxylation considerably enhances D08 binding affinity and contributes to cross‐reactivity among Ara h 1–3 allergens.
Parkkinen et al. (Tue,) studied this question.