A polyphasic taxonomic approach was employed to characterize two novel strains, P3C3 T and MAC6 T , isolated from sediments of the Innovation Way Drive Pond (Stillwater, OK) and the Cimarron River (Perkins, OK), respectively. Both strains were strictly aerobic, Gram-stain-negative, rod-shaped, motile and non-spore-forming bacteria. Strain P3C3 T grew optimally at 35 °C, pH 6.0 and 0.5% (v/w) NaCl, while strain MAC6 T grew optimally at 30 °C, pH 6.0 and 4.0% (v/w) NaCl. Based on 16S rRNA gene sequence similarity, P3C3 T was most closely related to Pseudomonas alcaligenes (98.3%), whereas MAC6 T was most closely related to Pseudomonas anguilliseptica (98.6%). Phylogenetic analysis using the 16S rRNA gene and whole-genome phylogenomic analysis indicated that P3C3 T and MAC6 T formed distinct branches within the Pseudomonas genus. Additionally, overall genomic-based relatedness indices demonstrated that P3C3 T and MAC6 T and the type strains of close relatives shared average nucleotide identity and digital DNA–DNA hybridization values <95% and <70%, respectively, which are below the currently accepted thresholds for species-level delineation. The genomes of strains P3C3 T and MAC6 T were 4.3 and 4.2 Mbp, respectively, with G+C contents of 64.5 and 60.5 mol%. Based on polyphasic taxonomic criteria, P3C3 T and MAC6 T represent two novel species within the genus Pseudomonas , for which the names Pseudomonas stagnisoli and Pseudomonas cimarronensis sp. nov. are proposed. The type strains are P3C3 T (=CCM 9468 T =CECT 31230 T =DSM 119661 T ) and MAC6 T (=CCM 9470 T =CCUG 78313 T =CECT 31231 T ), respectively.
Hasan et al. (Tue,) studied this question.