Quantification of Tissue Bacterial Load in a Mouse Model of Chronic Intestinal Infection

Key Points

• The research aims to quantify bacterial load in tissues affected by chronic Salmonella infection in a mouse model.
• Mouse model of chronic infection using streptomycin-resistant Salmonella typhimurium.
• Tissues were harvested post-euthanasia and sterile dissection.
• Homogenization of cecal and splenic tissues with steel beads followed by serial dilution.
• Bacterial colonies were counted after plating on selective agar.
• Quantification of bacterial load per gram of tissue from intestinal and systemic sites was achieved.
• Visible colonies indicated successful bacterial recovery from the tissues.